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Screening for autologous blood transfusions

Jul 8, 2009
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Does anyone know wheter they are planning to introduce this anytime soon?



The ratio between the amount of hemoglobin in the mature erythrocyte population and the reticulocytes (RBCHb:RetHb ratio) has previously been suggested as a marker to screen for EPO-abuse. We speculated that the reinfusion of blood would lead to a marked increase in this ratio, making it a valuable parameter in the screening for autologous blood doping. Three bags of blood (approximately 201+/-11 g of Hb) were withdrawn from 16 males and stored at either -80 degrees C (-80 T, n=8) or +4 degrees C (+4 T, n=8) and reinfused 10 weeks or 4 weeks later, respectively. Seven subjects served as controls. Different erythrocyte parameters were measured on a hematological analyzer serially throughout and during a 4 week wash-out period. By using RBCHb:RetHb ratio cut-off limits of 145.7 (1:100) ('suspicious') and 182.9 (1:1000) ('positive'), 35.4% (-80 T) and 19.6% (+4 T) of all samples obtained during a 4 week wash-out period were identified as 'suspicious', and 18.8% (-80 T) and 4.3% (+4 T) as 'positive'. In total, 7 out of 16 (43.8%) subjects had at least one sample exceeding 182.9. Compared to the currently used indirect parameters, the RBCHb:RetHb ratio is the best indicator of autologous blood doping after reinfusion, and the parameter could be used in a testing setting, once stability validation has been performed.

http://www.ncbi.nlm.nih.gov/pubmed/...nel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
 
Jun 16, 2009
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seeing the way things went with the EPO test this will not be introduced until it has been delayed long enough for the best riders to find a new way to maintain the status quo of the current pecking order.

Then when it is introduced a few lesser names from the continental teams will be sacrificed in the name of a clean sport.
 
Mar 14, 2009
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Junk/incomplete Science?

I hope the test is refined/further validated, as if it was used now, 43.8% of autologous blood transfusions would be detected!!

Also, the trial only used 16 subjects. How many of those were females, what ages were the subjects, & how many ethnicities were tested? Do the limits need to be varied due to any of these?

Reading more than just the abstract would be more informative too.
 
Aug 17, 2009
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This has been used since 2004 in cycling. It is the entire foundation for the passport system. Note your cut off scores of 143 and 189... In the Passport system a score of over 134 is 'positive'. So, its actually much stricter than what is looked at here. The released Wiggins results never exceed 100.

JV
 
Aug 9, 2009
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JV1973 said:
This has been used since 2004 in cycling. It is the entire foundation for the passport system. Note your cut off scores of 143 and 189... In the Passport system a score of over 134 is 'positive'. So, its actually much stricter than what is looked at here. The released Wiggins results never exceed 100.

JV

No. You mix up many things.

Firstly, you confuse the stimulation index called OFF-score and the ratio between the hemoglobin of mature cells and hemoglobin of immature cells. Two very different markers of doping.

Secondly, the OFF-score does not represent "the entire foundation of the passport". The OFF-score is one marker among others in the passport. Nothing else.

Finally, there is no cut off at 134 anymore, but individual cut off scores estimated with the information stored in the passport. This is the fundamental principle of the passport.

Not encouraging that a DS does not even know the basis of the passport.