Test to detect transfusions from DEHP-free blood bags

May 19, 2010
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The plasticiser test was a roaring sucsess for the producers of blood bags not containing DEHP. No one has been caught by it, except Contador, who also slipped away. Now a study is done to look for biomarkers which can be used to detect prohibited autologous transfusions using DEHP-free blood bags, to level the field for blood bag producers. ;)

SN: Can you tell us more about the study that you are carrying out?
NL: We will be testing volunteers who have received autologous transfusions from either DEHP or DEHP-free blood bags. We will be testing them at different times, and using different matrices (serum, plasma, urine) to look for a variety of biomarkers that we believe could be used for detection of transfusions with DEHP-free blood bags.

When blood is stored in blood bags, several molecular changes occur in the red blood cells. These changes are known as ‘storage legions’. Autologous transfusion of these stored red cells can cause inflammation and increase the risk of infection. It has also been shown that transfusion with stored red cells produces extravascular hemolysis. Extravascular hemolysis causes an increase in total bilirubin and an increase of circulating non-transferrin-bound iron. This in turn produces inflammation and results in an increase of secreted proteins such as interleukin-6 (IL-6).

Circulating micro RNAs (miRNA) are also promising biomarkers for detecting physiological responses to any immune response resulting from autologous transfusion. miRNAs are stable and sensitive so they have the potential to serve as long-term biomarkers. These markers could be used in the blood doping field, but also for the detection of various diseases and injuries. We have previously shown that circulating miRNAs can be used to detect autologous blood transfusion with DEHP-blood bags. In this study, we will look at the presence of miRNAs in volunteers who have received a DEHP-free blood transfusion.
http://www.selectscience.net/product-news/Exclusive-Blood-Doping-Interview--Detecting-Autologous-Blood-Transfusion-with-Clinical-Biomarkers/?artID=36419&compname=SelectScience
 
When blood is stored in blood bags, several molecular changes occur in the red blood cells. These changes are known as ‘storage legions’
"lesions", not "legions". Also:

During a transfusion, significant amounts of DEHP leech into the bloodstream
I hope not! DEHP "leaches" into the bloodstream.

Several labs have been working on this for quite a while. No question that changes like this occur. The question is how reproducible they are in different individuals, and whether they can be clearly distinguished from changes resulting from certain diseases. IOW, is this going to be a clear-cut doping test, or a biopassport-style result that requires a panel of experts to decide on.
 
D-Queued said:
sniper said:
to level the field for blood bag producers.
:D
though as far as i know the plasticizer test was never validated.


"lesions", not "legions".
:D
It was validated by Contador, just not treated as a separate doping violation.

But, he confirmed what we already knew and it is now immortalized for all time. There is some level of justice in that.

Dave.
It was never legally validated which is what sniper meant I think.
 
LaFlorecita said:
...
It was never legally validated which is what sniper meant I think.
Understood, but sometimes we can do better than that.

And, in the case of Aldirto Contadope (love your sig btw), we 'know' conclusively that the current poster boy is as tainted as any Texan could aspire.

Dave.
 
Aug 10, 2010
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Alpuerto Clenbutador is now well squared away with a proper blood bag manufacturer.
 

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