Cobble, thanks, I was hoping someone else would pick up the ball here. I thought about having more time for the body to adjust to the transfusion, but was not sure exactly what that means. Does a rider have any ill or odd effects immediately after a transfusion which dissipate over a day or two? If so, then it makes sense to do it a little ahead of time.
Wrt plasma infusion, again, I have been missing something. I think what WADA is proposing, according to El Pais, is a plasma storage involving dessication. IOW, it is stored as a powder, easily transportable, which can be quickly reconstituted with distilled water. This makes it somewhat easier to understand a second transfusion. Bert wanted to reduce his HT further on July 21, so instead of using saline, he just added water to the powder and put that in. In addition to the salts present in normal saline, there would be other salts and numerous other substances that would have a beneficial effect. Not particularly PE, but restorative in the same sense that a saline infusion would be, probably a little more. In any case, with the powder, it would be even easier to do than saline, as all you need is sterile water.
(Keep in mind, Bert would not even have to transfuse the reconstituted plasma. He could drink it, just the way you would drink a salt solution. Some of the beneficial substances would be degraded in the digestive system, but some would be absorbed, and it would be perfectly legal to do this, AFAIK. However, drinking would not lower the HT as effectively as transfusion would).
To summarize: transfusion of red cells on July 19 or 20, followed by a second transfusion of plasma on July 21. The second transfusion I believe would have been planned all along, as a way of further lowering the HT. This being the case, it would have made sense to save the dried plasma, rather than use it to reconstitute the red cells. Saline was used for the latter.
Are you sure the July 20 sample was not analyzed in Cologne? I thought that was only speculation. If it was analyzed somewhere else, and it turns out it would have been positive, then as noted earlier, it’s possible Bert took CB before the Tour and his positives reflect that and not transfusion. I have made a rough calculation that for Bert to pass all CB tests beginning with the start of the Tour, at 2 ng/ml, the half-life of the second slower clearance of CB from the body would have to be on the order of eight days. I don’t know what it actually is. In cattle it has been estimated as 3 days, but the initial clearance in cattle is much faster than in humans, 10 hr vs. 35 hr. So this scenario might be possible.
Alternatively, as you say, Bert might have transfused on July 19/20. Then there is no need to bring in a second transfusion of plasma alone. It may be that WADA is pursuing both this scenario and the one where there is a second transfusion. The reason I think they might is because the calculated CB dose reported, 200 ug, is higher than necessary to explain 50 pg/ml if transfusion took place on July 21, but might be necessary if transfusion took place a day earlier. OTOH, if WADA were pursuing this possibility, they would surely have tested the July 20 sample at Cologne by now, and would not even be considering the alternative scenario where plasma is infused the following day.
Edit:
i am growing more confident that 20 july sample was tested by cologne. i provided the reasoning earlier.
If this is correct, then we are down to just the second, plasma transfusion. This would be what WADA is hanging its hat one.
Now I think the remaining pieces of the puzzle fall in place:
1) different times at which DEHP and CB entered Bert’s system: check
2) transfusion of red cells before the rest day: check
3) second transfusion of plasma alone: check
Sure, it’s a little more involved than ideal, it would be more plausible if DEHP and CB entered the body at the same time, but it has a lot of the elements you would expect:
1) off-season PED use
2) off-season blood withdrawal
3) use of high tech storage procedures to avoid/reduce need for withdrawal-transfusion cycles